LOCUS       sequence-78037-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRb11_3726
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     115..133
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(299..327)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca
       61 gctggcaatt ccgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
      121 cgtatcacga ggccctttcg tcttcacctc gaggcctcca caccgctcgt cacatcctgt
      181 gatccactct tcatcccgct acgtaacacc tctgcatcgc gaaccaaaac cagggatcct
      241 gtcaatttcc gcgatagagg aggtaaagat gagcaaagga gaagaacttt tcactggagt
      301 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtccgtgg
      361 agagggtgaa ggtgatgcta caaacggaaa actcaccctt aaatttattt gcactactgg
      421 aaaactacct gttccgtggc caacacttgt cactactctg acctatggtg ttcaatgctt
      481 ttcccgttat ccggatcaca tgaaacggca tgactttttc aagagtgcca tgcccgaagg
      541 ttatgtacag gaacgcacta tatctttcaa agatgacggg acctacaaga cgcgtgctga
      601 agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaagggta ttgattttaa
      661 agaagatgga aacattcttg gacacaaact cgagtacaac tttaactcac acaatgtata
      721 catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc gccacaacgt
      781 tgaagatggt tccgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg
      841 ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgtccttt cgaaagatcc
      901 caacgaaaag cgtgaccaca tggtcctt
//
