LOCUS       sequence-78139-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  baculovirus-based delivery system that enables the efficient 
            incorporation of unnatural amino acids into proteins in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Chatterjee A, Xiao H, Bollong M, Ai HW, Schultz PG
  TITLE     Efficient viral delivery system for unnatural amino acid mutagenesis
            in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. doi: 
            10.1073/pnas.1309584110. Epub 2013 Jul 1.
  PUBMED    23818609
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1309584110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-16- 16"; volume: "110"; issue: "29"; pages: "11803-8"
COMMENT     Sequence Label: pAcBac1.tR4-MbPyl
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agctggctag cgccaccatg gataaaaaac cattagatgt tttaatatct gcgaccgggc
      121 tctggatgtc caggactggc acgctccaca aaatcaagca ccatgaggtc tcaagaagta
      181 aaatatacat tgaaatggcg tgtggagacc atcttgttgt gaataattcc aggagttgta
      241 gaacagccag agcattcaga catcataagt acagaaaaac ctgcaaacga tgtagggttt
      301 cggacgagga tatcaataat tttctcacaa gatcaaccga aagcaaaaac agtgtgaaag
      361 ttagggtagt ttctgctcca aaggtcaaaa aagctatgcc gaaatcagtt tcaagggctc
      421 cgaagcctct ggaaaattct gtttctgcaa aggcatcaac gaacacatcc agatctgtac
      481 cttcgcctgc aaaatcaact ccaaattcgt ctgttcccgc atcggctcct gctccttcac
      541 ttacaagaag ccagcttgat agggttgagg ctctcttaag tccagaggat aaaatttctc
      601 taaatatggc aaagcctttc agggaacttg agcctgaact tgtgacaaga agaaaaaacg
      661 attttcagcg gctctatacc aatgatagag aagactacct cggtaaactc gaacgtgata
      721 ttacgaaatt tttcgtagac cggggttttc tggagataaa gtctcctatc cttattccgg
      781 cggaatacgt ggagagaatg ggtattaata atgatactga acttt
//
