LOCUS       sequence-78144-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  baculovirus-based delivery system that enables the efficient 
            incorporation of unnatural amino acids into proteins in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Chatterjee A, Xiao H, Bollong M, Ai HW, Schultz PG
  TITLE     Efficient viral delivery system for unnatural amino acid mutagenesis
            in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. doi: 
            10.1073/pnas.1309584110. Epub 2013 Jul 1.
  PUBMED    23818609
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1309584110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-16- 16"; volume: "110"; issue: "29"; pages: "11803-8"
COMMENT     Sequence Label: pAcBac1.tR4-MbPyl
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(175..269)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    175..193
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    203..221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(239..258)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        complement(346..594)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(404..423)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(574..594)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     polyA_signal    627..761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(651..670)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     705..724
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gggaactggg tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga
       61 actagatttc acttatctgg ttcggatctc ctagcaaaaa acggggggtg cgggagtttc
      121 acccgcctgc acggatttag agtccgtgtg atctattcga tccacccccc ggtgtctcta
      181 tcactgatag ggaacttata agtctctatc actgataggg atttcacgtt tatggtgatt
      241 tcccagaaca catagcgaca tgcaaatatt aaaaaacgga aaccccggga atctaacccg
      301 gctgaacgga tttagagtcc gttcgatcta catgatcagg tttccggtgt ttcgtccttt
      361 ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata
      421 gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg
      481 tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa ttatctctct
      541 aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcttcctg
      601 cccgacctag gctcaagcag tgatcagatc cagacatgat aagatacatt gatgagtttg
      661 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      721 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
      781 attttatgtt tcangttcag ggggangtgt gggaggtttt ttaaagcaag taaaacctct
      841 acaaatgtgg tatggctgat tatgatcctc tagtacttct cgacaagctt ggccggcctg
      901 g
//
