LOCUS       sequence-78182-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid separately encodes a human codon-optimized SpCas9 
            nickase, a tracrRNA and customizable crRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: 
            pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..91
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtttaatta cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac
       61 catgtaccca tacgatgttc cagattacgc ttcgccgaag aaaaagcgca aggtcgaagc
      121 gtccgacaag aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt
      181 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
      241 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
      301 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
      361 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
      421 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
      481 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
      541 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
      601 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
      661 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
      721 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
      781 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggcaa
      841 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
      901 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
      961 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     1021 cctgctgagc gacatcctga gagtgaacac cga
//
