LOCUS       sequence-78188-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Huang HH, Camsund D, Lindblad P, Heidorn T
  TITLE     Design and characterization of molecular tools for a Synthetic 
            Biology approach towards developing cyanobacterial biotechnology.
  JOURNAL   Nucleic Acids Res. 2010 May . 38(8):2577-93.
  PUBMED    20236988
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2010 May . 38(8):2577-93."
COMMENT     Sequence Label: pPMQAK1
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(41..98)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    99..119
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             461..766
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     645..664
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    795..816
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gcgagtcagt gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa
       61 aagggcaggg tggtgacacc ttgccctttt ttgccggact gcagcggccg ctactagtaa
      121 cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
      181 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
      241 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
      301 gatgtcaata tctccggtct ggtaagcgca accatgcaga atgaagcccg tcgtctgcgt
      361 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
      421 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
      481 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
      541 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
      601 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
      661 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
      721 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
      781 tatacacagc cagtctctag aagcggccgc gaattccaga aatcatcctt agcgaaagct
      841 aaggattttt tt
//
