LOCUS       sequence-78343-e         586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi 
            MZ, Mukhopadhyay A
  TITLE     Engineering microbial biofuel tolerance and export using efflux 
            pumps.
  JOURNAL   Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
  PUBMED    21556065
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2011-05-10"; volume: "7:487. doi"; pages: " 
            10.1038/msb.2011.21"
COMMENT     Sequence Label: pBbA5k-EPL84
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtggtgatgg tgatgatgtg gttgcttcgt cgttgattcc tgccagccgc cgccgagcgc
       61 cttgtagagt tcgacgcggt tgatcaatgc cgccagcccg gtctgcncca gcgcgagctg
      121 cgcgttgaag aagtcgacct gggccgtctg cacctgcaga tagctgtcga tgccattgtt
      181 gtagcgcagg ttggacagct ccagcgtgcg cccggccgcc acctgcaacg cgcgctgcgc
      241 gtcgagctgc gcgctgtagg tcgcctcgcc ggccagcgta tccgacacct cgcggaacgc
      301 ttgctggatg ctctgttcgt actgggccac cgcgatgttg tcgcgcgcct tggccaggtt
      361 caggccttcg cggatgctgc cgccggcgaa caggggcgtg gtgatcgacg gcgagaagct
      421 ccaatagccc tgcccgccct tgaacagggt gtccaacgac gtgctggcca cgcccaacag
      481 gccggtcagc gaaatggtcg ggaagaacgc cgcgcgcgcc gcgccgatat tggcattggc
      541 cgccttgagc tggttctccg ccgccaggat gtcgggccgg cgcgtc
//
