LOCUS       sequence-78440-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with canonical 
            C-terminal domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-CtermCan
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..48
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             55..75
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtgattata aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag
       61 aagaagagga aggtgggcat tcaccgcggg gtacctatgg tggacttgag gacactcggt
      121 tattcgcaac agcaacagga gaaaatcaag cctaaggtca ggagcaccgt cgcgcaacac
      181 cacgaggcgc ttgtggggca tggcttcact catgcgcata ttgtcgcgct ttcacagcac
      241 cctgcggcgc ttgggacggt ggctgtcaaa taccaagata tgattgcggc cctgcccgaa
      301 gccacgcacg aggcaattgt aggggtcggt aaacagtggt cgggagcgcg agcacttgag
      361 gcgctgctga ctgtggcggg tgagcttagg gggcctccgc tccagctcga caccgggcag
      421 ctgctgaaga tcgcgaagag agggggagta acagcggtag aggcagtgca cgcctggcgc
      481 aatgcgctca ccggggcccc cttgaacctg accccagacc aggtagtcgc aatcgcgtca
      541 aacggagggg gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa
      601 gaccacggcc ttacaccgga gcaagtcgtg gccattgcat cccacgacgg tggcaaacag
      661 gctcttgaga cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc
      721 gatcaagttg tagcgattgc gtcgaacatt ggagggaaac aagcattgga gactgtccaa
      781 cggctccttc ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc
      841 gcctcgaatg gcggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg
//
