LOCUS       sequence-78479-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-CtermQ3
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..629)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 aggagagggt tagggatagg cttaccttcg aagggccctt aaaagtttat ctcgccgtta
       61 ttaaatttcc gtctcacttc ctctaaggtt aatgtgccgg ctttaatcat ttctccacca
      121 attaaaagct cttctacact aagaacagct ccattacaat tagtcttatg atttaatcgt
      181 gtaagctgag ctttgtagtt tcctttaaag tgaccactca caaataaaaa cttaaattcc
      241 gttacagaag atggatagac tttccaccat tcattagggt tgatatgttt gtttcgtgtt
      301 tgattttctt tgacatatcg ttgcatttca tctgcttggc caattggcag attataacct
      361 ccgctataag ctttagtatc cacgatcaca ccgtaatcaa taggagatcc gacagtataa
      421 attgctccgt ccggtttcct tgatccaccc aaatgtttac ctctatatcc ataaactttc
      481 ataaaaaatt ccattacctt catttcaaga attctatcct gagtggaatt tctggcaatt
      541 tcaattaatt caatatattc atgaggcaca tatttcaatt tatgacgaag ttcagatttc
      601 ttctcctcca gttcactttt gactagttgg gatcccgcga cttgatggga agttctctcg
      661 ggaatccgtt ggttggttct ttggatcaat gcgggagcat gaggcagacc ctttttgact
      721 gcatcgagcg cgggtagtcc accaagacat gccaacgcca ccagatggta attcgttacg
      781 cagccaacgc gggatcgggc ctggacaatt gggctacgat ggactccaag
//
