LOCUS       sequence-78481-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-CtermQ7
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..70
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             77..97
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggnnacnnnn nnnnaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
       61 cgatgacaag atggccccca agaagaagag gaaggtgggc attcaccgcg gggtacctat
      121 ggtggacttg aggacactcg gttattcgca acagcaacag gagaaaatca agcctaaggt
      181 caggagcacc gtcgcgcaac accacgaggc gcttgtgggg catggcttca ctcatgcgca
      241 tattgtcgcg ctttcacagc accctgcggc gcttgggacg gtggctgtca aataccaaga
      301 tatgattgcg gccctgcccg aagccacgca cgaggcaatt gtaggggtcg gtaaacagtg
      361 gtcgggagcg cgagcacttg aggcgctgct gactgtggcg ggtgagctta gggggcctcc
      421 gctccagctc gacaccgggc agctgctgaa gatcgcgaag agagggggag taacagcggt
      481 agaggcagtg cacgcctggc gcaatgcgct caccggggcc cccttgaacc tgaccccaga
      541 ccaggtagtc gcaatcgcgt cacatgacgg gggaaagcaa gccctggaaa ccgtgcaaag
      601 gttgttgccg gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg tggccattgc
      661 aagcaatggg ggtggcaaac aggctcttga gacggttcag agacttctcc cagttctctg
      721 tcangcccac gggctgactc ccgatcaagt tgtancgatt gcgtccaacg gtggagggaa
      781 acaagcattg nagactgtcc aacggctcct tcccgtgttg tgtcnagccc acggtttgac
      841 gcctgca
//
