LOCUS       sequence-78484-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-CtermQ7
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..45)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(61..648)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 acgcgtagaa tcgagaccga ggagagggtt agggataggc ttaccttcga agggccctta
       61 aaagtttatc tcgccgttat taaatttccg tctcacttcc tctaaggtta atgtgccggc
      121 tttaatcatt tctccaccaa ttaaaagctc ttctacacta agaacagctc cattacaatt
      181 agtcttatga tttaatcgtg taagctgagc tttgtagttt cctttaaagt gaccactcac
      241 aaataaaaac ttaaattccg ttacagaaga tggatagact ttccaccatt cattagggtt
      301 gatatgtttg tttcgtgttt gattttcttt gacatatcgt tgcatttcat ctgcttggcc
      361 aattggcaga ttataacctc cgctataagc tttagtatcc acgatcacac cgtaatcaat
      421 aggagatccg acagtataaa ttgctccgtc cggtttcctt gatccaccca aatgtttacc
      481 tctatatcca taaactttca taaaaaattc cattaccttc atttcaagaa ttctatcctg
      541 agtggaattt ctggcaattt caattaattc aatatattca tgaggcacat atttcaattt
      601 atgacgaagt tcagatttct tctcctccag ttcacttttg actagttggg atcccgcgac
      661 ttgatgggaa gttctctcgg gaatttgttg gttggtttgt tggatcaatg cgggagcatg
      721 aggcagacct tgttggactg catcgagcgc gggtcgtcca ccaagacatg ccaacgccac
      781 cagatggtca ttcgttaacg cagccaacgc gggatcgggc ctggacaatt gggctacgat
      841 ggactccaag
//
