LOCUS       sequence-78540-e        6763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deletes the cpc operon in Synechocystis and confers kanamycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Kirst H, Formighieri C, Melis A
  TITLE     Maximizing photosynthetic efficiency and culture productivity in 
            cyanobacteria upon minimizing the phycobilisome light-harvesting 
            antenna size.
  JOURNAL   Biochim Biophys Acta. 2014 Oct;1837(10):1653-64. doi: 
            10.1016/j.bbabio.2014.07.009. Epub 2014 Jul 18.
  PUBMED    25046143
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbabio.2014.07.009"; journalName: "Biochim Biophys Acta";
            date: "2014-10"; volume: "1837"; issue: "10"; pages: "1653-64"
COMMENT     Sequence Label: pJ207 Delta-cpc-KanR
FEATURES             Location/Qualifiers
     source          1..6763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             427..960
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      1082..1670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1571..1590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1824..1841
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2974..3789
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4543..4562)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        4629..4720
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4939..4958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             5565..6374
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6455..6474)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agggtttatt gagaatatgt ttttcgtcag cgccaatccc tgggtgagtt tcaccagttt
       61 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacta tgggcaaata
      121 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg
      181 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
      241 gggcggggcg taaacgccga ggaggaaaaa aaaatgcgct cacgcaactg gtccagaacc
      301 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
      361 ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
      421 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
      481 ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga ccaagtcaaa
      541 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gtgacgtagc cacctactcc
      601 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac attcatcgcg
      661 cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt tctgcccaag
      721 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg cgagcaccgg
      781 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
      841 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
      901 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
      961 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     1021 gagttacgcg cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1081 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1141 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1201 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta gcccaccact
     1261 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1321 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1381 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1441 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1501 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1561 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1621 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1681 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1741 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1801 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ggcgagagta
     1861 gggaactgcc aggcatcaaa ctaagcagaa ggcccctgac ggatggcctt tttgcgtttc
     1921 tacaaactct ttctgtgttg taaaacgacg gccagtctta agctcgggcc ccctgggcgg
     1981 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
     2041 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaagggcg cctgtcactt
     2101 tgcttgatat atgagaatta tttaacctta taaatgagaa aaaagcaacg cactttaaat
     2161 aagatacgtt gctttttcga ttgatgaaca cctataatta aactattcat ctattattta
     2221 tgattttttg tatatacaat atttctagtt tgttaaagag aattaagaaa ataaatctcg
     2281 aaaataataa agggaaaatc agtttttgat atcaaaatta tacatgtcaa cgataataca
     2341 aaatataata caaactataa gatgttatca gtatttatta tgcatttaga ataaattttg
     2401 tgtcgccctt cgctgaaaag cttaagagtc cctgaatatc aaaatggtgg gataaaaagc
     2461 tcaaaaagga aagtaggctg tggttcccta ggcaacagtc ttccctaccc cactggaaac
     2521 taaaaaaacg agaaaagttc gcaccgaaca tcaattgcat aattttagcc ctaaaacata
     2581 agctgaacga aactggttgt cttcccttcc caatccagga caatctgaga atcccctgca
     2641 acattactta acaaaaaagc aggaataaaa ttaacaagat gtaacagaca taagtcccat
     2701 caccgttgta taaagttaac tgtgggattg caaaagcatt caagcctagg cgctgagctg
     2761 tttgagcatc ccggtggccc ttgtcgctgc ctccgtgttt ctccctggat ttatttaggt
     2821 aatatctctc ataaatcccc gggtagttaa cgaaagttaa tggagatcag taacaataac
     2881 tctagggtca ttactttgga ctccctcagt ttatccgggg gaattgtgtt taagaaaatc
     2941 ccaactcata aagtcaagta ggagattaat tcaatgagtc acatccagag agaaactagt
     3001 tgttcccgac ctcgtttgaa tagcaatatg gatgcagatc tgtacggata taaatgggcg
     3061 cgagataacg taggccaatc tggggccact atttatcggt tatatggcaa accagatgct
     3121 cccgaactgt ttctcaaaca tggcaaaggg tctgtggcca atgatgttac cgatgaaatg
     3181 gtgcggttga actggttgac agaatttatg cccctcccga ccatcaaaca ttttatcagg
     3241 actccagacg atgcatggct attaactacg gccattcctg ggaaaactgc ctttcaggtg
     3301 ttggaagaat atcccgattc tggtgagaat atcgtcgatg cgttagcggt ttttctaaga
     3361 cgtctacata gcattcccgt ttgcaattgt ccctttaatt cggaccgggt gttccgcttg
     3421 gcgcaggctc agtcccggat gaataacggt ttggtagatg cctcggactt tgatgatgaa
     3481 cggaacggct ggcccgttga acaggtttgg aaagagatgc ataagctgct gcccttctcc
     3541 cccgacagcg ttgttactca tggagatttt tctctcgata atctgatttt cgacgaaggc
     3601 aagctaattg gctgtatcga tgtgggacgg gtagggattg cggaccggta tcaagaccta
     3661 gcaattttgt ggaactgcct aggtgaattt tcccccagcc tacaaaaacg gctgtttcaa
     3721 aaatacggaa tcgataatcc cgacatgaac aaattacaat ttcatctgat gctagatgag
     3781 ttcttttagg ctattttgtt aattactatt tgagctgagt gtaaaatacc ttacttactc
     3841 aaaagcatta actaaccata acaatgacta atctcttttt ttgattgaac tccaaactag
     3901 aatagccatc gagtcagtcc atttagttca ttattagtga aagtttgttg gcggtgggtt
     3961 atccgttgat aaaccaccgt ttttgtttgg gcaaagtaac gatttgatgc agtgatgggt
     4021 ttaaagataa tcccgtttga ggaaatcctg caggacgacg ggaactttaa cctgaccgct
     4081 gctgggttcg taataatttt ctaaaattgc cgccatggtg cgcccgatcg ccaaaccgga
     4141 accgttgaga gtgtgaacaa attgggtgcc ttttttgccc ttttccttgt agcgaatgtt
     4201 ggcccgacgg gcttggaaat cgtggaagtt agaacaactg gaaatttccc ggtaggtgtt
     4261 agccgatggt aaccaaactt ccaagtcgta gcatttagcc gctccaaaac ctaaatcacc
     4321 ggtacataat tccaccactg agctccgtca aaagggcgac acccctcaat tagcccgggc
     4381 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctact
     4441 ctcgcatggg gagtccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc
     4501 atggggtcag gtgggaccac cgcgctactg ccgccaggca aacaaggggt gttatgagcc
     4561 atattcaggt ataaatgggc tcgcgataat gttcagaatt ggttaattgg ttgtaacact
     4621 gacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4681 accctgataa atgcttcaat aatattgaaa aaggaagaat atgagtattc aacatttccg
     4741 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4801 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4861 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4921 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4981 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5041 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5101 gagtgataac actgcggcca acttacttct gacaacgtgc acaacatggg ggatcatgta
     5161 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5221 accacgatgc ctgtagcgat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5281 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5341 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatccgg agccggtgag
     5401 cgtggttctc gcggtatcat cgcagcgctg gggccagatg gtaagccctc ccgtatcgta
     5461 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5521 ataggtgcct cactgattaa gcattggtaa aggaggaaaa aaaaatgagc catattcaac
     5581 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     5641 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     5701 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     5761 agatggtcag actaaactgg ctgacggaat ttatgccact tccgaccatc aagcatttta
     5821 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcgttcc
     5881 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     5941 tgcgccggtt gcactcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     6001 gcctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     6061 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     6121 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     6181 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     6241 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     6301 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     6361 atgagttttt ctaaaggagg aaaaaaaaat ggagaaaaaa atcactggat ataccaccgt
     6421 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     6481 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     6541 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     6601 ggagttccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     6661 ttacaccgtt ttccatgagt gcacacgacg atttccggca gtttctacac atatattcgc
     6721 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taa
//
