LOCUS       sequence-78549-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of human codon-optimized Cas9 nuclease and GFP, 
            plasmid optimized for expression in human pluripotent stem cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Ding Q, Regan SN, Xia Y, Oostrom LA, Cowan CA, Musunuru K
  TITLE     Enhanced efficiency of human pluripotent stem cell genome editing 
            through replacing TALENs with CRISPRs.
  JOURNAL   Cell Stem Cell. 2013 Apr 4;12(4):393-4. doi: 
            10.1016/j.stem.2013.03.006.
  PUBMED    23561441
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.03"; journalName: "Cell Stem Cell"; date: 
            "2013-04-4- 4"; volume: "12"; issue: "4"; pages: "393-4"
COMMENT     Sequence Label: pCas9_GFP
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(97..108)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag ctgccgccaa taatttttca
       61 ttttcttgac aacttcttct gaggggacgt tatcactctt ccctctattt ttatcggatc
      121 ttgtcaacac tttattatca atagaatcat ctttgagaaa agactggggc acgatatgat
      181 ccacgtcgta gtcggagagc cgattgatgt ccagttcctg atccacgtac atgtccctgc
      241 cgttctgcag gtagtacagg tagagcttct cattctgaag ctgggtgttt tcaactgggt
      301 gttccttaag gatttgggac cccagttctt ttataccctc ttcaatcctc ttcatccttt
      361 ccctactgtt cttctgtccc ttctgggtag tttggttctc tcgggccatc tcgataacga
      421 tattctcggg cttatgcctt cccattactt tgacgagttc atccacgacc ttaacggtct
      481 gcagtattcc ctttttgata gctgggctac ctgcaagatt agcgatgtgc tcgtgaagac
      541 tgtccccctg gccagaaact tgtgctttct ggatgtcctc cttaaaggtg agagagtcat
      601 catggatcaa ctgcatgaag ttccggttgg caaatccatc ggacttaaga aaatccagga
      661 ttgtctttcc actctgcttg tctcggatcc cattgatcag ttttcttgac agccgccccc
      721 atcctgtata tcggcgcctc ttgagctgtt tcatgacttt gtcgtcgaag agatgagcgt
      781 aagttttcaa gcgttcttca atcatctccc tatcttcaaa caacgtaagg gtgaggacaa
      841 tgtcctcaag aatgtcctcg ttctcctcat tgtcc
//
