LOCUS       sequence-78552-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of human TPI1 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Gruning NM, Du D, Keller MA, Luisi BF, Ralser M
  TITLE     Inhibition of triosephosphate isomerase by phosphoenolpyruvate in 
            the feedback-regulation of glycolysis.
  JOURNAL   Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232.
  PUBMED    24598263
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Open 
            Biol."; date: "2014-03-5"; volume: "4(3):130232. doi"; pages: " 
            10.1098/rsob.130232"
COMMENT     Sequence Label: pET20b- hTPI
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..45)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             809..831
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(862..881)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(863..881)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcgggctttg ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gttgtttggc
       61 attgatgatg tccacgaatt cgggcttgag ggaagcacca cccacaagga agccatccac
      121 atcaggctgg ctggccagct ccttgcaggt tgccccagtc acagagcctc cataaatgat
      181 acgggtgctc tgagccaccg catcagagac gttggacttc agccatcctc ggagcttctc
      241 gtgtacttcc tgggcctgtt ggggtgttgc agtcttgcca gtaccaatgg cccacacagg
      301 ctcataggcc aggacgacct tgctccagtc cttcacgtta tctgcgatga cctttgtctg
      361 ctcgaaaaca accttctcag tgatgccagc ttccctttca tctagcttct ccccaatgca
      421 ggcgattact ccgagtccct ctgccagagc atgggccact ttctgcccaa tcagctcatc
      481 tgactcccca aagacatgcc ttctctctga gtgccccagg accacccacg tggctccgca
      541 gtctttgatc atgccagggc tgatctcccc agtaaaagcc ccattagtca ctttgtagca
      601 gttctgcgca gccacagcaa tcttgggatc tagcttctgc cgggcgaagt cgatataggc
      661 agtaggggga gcacaaacca cctcggtgtc ggccggtacc ttggccgcgt tcagagtgcc
      721 gatgagctcc cccagactct gcttccgccc gttcatcttc cagtttcccc caacgaagaa
      781 cttcctggag ggcgccatca tatgtatatc tccttcttaa agttaaacaa aattatttct
      841 agagggaaac cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc
      901 tcgggcagcg ttgggtc
//
