LOCUS       sequence-78584-e        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for
            DSB mediated EGFP reconstitution. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M
  TITLE     Generation of mutant mice by pronuclear injection of circular 
            plasmid expressing Cas9 and single guided RNA.
  JOURNAL   Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355.
  PUBMED    24284873
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-27"; volume: "3:3355. doi"; pages: " 
            10.1038/srep03355"
COMMENT     Sequence Label: pCAG-EGxxFP-Cetn1
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     304..323
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
       61 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      121 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
      181 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      241 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
      301 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
      361 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
      421 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
      481 gtgcagatga acttcagggt cagcttgccg atatcgaatt ctgcaggcgc ataggggctg
      541 gcgcaggcgc actagcaggg cacctcacac acgtgcgatg tcagggcacc gttaagatcc
      601 caactgttgt ggaggatggc gtccaccttc aggaagtcaa acgttgcctc taccagctac
      661 aagagaaagg tgggtcctaa gcctgaactc accgaagatc aaaagcaaga agttcgggaa
      721 gcctttgacc tcttcgattc tgatgggagc gggaccatcg atgtgaagga gctgaaggtg
      781 gccatgagag cacttggctt tgaacccagg aaggaagaga tgaagaaaat gatttcagaa
      841 gtagacaaag aggccacagg aaagatcagc ttcaatgact tcttggctgt gatgactcag
      901 aagatggccg agaaagatac caaagaggaa atcctgaagg ctttcaggtt gtttgatgac
      961 gacgaaactg ggaaaatttc attcaaaaac ctcaagcgtg tggccaatga gctgggggaa
     1021 agcctcacag acgaggagct gcaggaaatg attgatgaag ctgatcgtga tggcga
//
