LOCUS       sequence-78607-e         457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pCDM4-GLY
FEATURES             Location/Qualifiers
     source          1..457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgagtt
       61 acttcttctt cgctttcggg ttcggcaggt cggtaatgct accttcgaac acttctgccg
      121 ccaggcccac agactcgtgc agagtcgggt gcgcgtggat ggtcagtgcg atgtcttcag
      181 catcacaacc catttcgatt gccaggccga tttcacccag cagctcgccg ccgttagtac
      241 cgacaatcnc nccaccgatc acacngtgag attctttgtc naaaatcagc ttggtcatac
      301 cgtctgcgca gtcngaagcg atagcacgac cagaagcagc ccacgggaag gtggcggttt
      361 catanctgat gcctttctct ttcgcttctt tctcagtcag acccacccat gcaacttctg
      421 gntcggtata ggcgatggac gggataactt tcggatc
//
