LOCUS       sequence-78608-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length MSP3.5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: MSP3.5-bio-His
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     202..221
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    220..275
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(327..344)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acccctttgt gtttggtccc cccccgtgtc acatgtggaa cagggcccag ttggcaagtt
       61 gtaccaacca actgaaggga ttacatgcac tgccccgcga agaaggggca gagatgtcgt
      121 agtcaggttt agttcgtccg gggcggggat cgatcctcta gctagagatc tccgagggat
      181 ctcgaccaaa tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca
      241 acacactatt gcaatgaaaa taaatttcct ttattagcca gaggtcgagg tcgggggatc
      301 cttaattaat cacgtgtcta gactcaatgg tggtgatggt ggtgggggcc cagaggggcc
      361 agaggaggca ggttcctgtc cctgtggttc cacagcatct tctgggcgtc caggatgtgg
      421 tgcaggctgc cggaattcaa ccctttggat aaaacctgga tcttggagtc catcttgacc
      481 tcttcacctt cactcagtag acattgccac acccctgctt caggggcctg cacttgaatc
      541 actttctcct gcctggagac cctggcctcc tgattctcct gcttcaagat cagtctcatc
      601 ttgggtgagg tgggtcccat cacctcacag gtcaaagtgt tgctgtcggg ctgagtcact
      661 ttcatcacca ccaggttcac ttcctgatac agtatccctc tgtccagagt cagggtcagg
      721 ttgccagaac cagcaaactg aagggagacc tggggtatct gaagggtgag tgggagcgtt
      781 tcggacagct ggaacttggg gttgctagta gacttctgca cagacacctt ttggttcttt
      841 a
//
