LOCUS       sequence-78631-e        6890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express Cre in neurons 
            from the synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV pmSyn1-EBFP-Cre
FEATURES             Location/Qualifiers
     source          1..6890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     regulatory      1796..1805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1802..1804,1805..1807,1808..2518)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     CDS             2531..3562
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    3637..4113
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3836..3855)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4153..4293
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4153..4282
                     /label=AAV2 ITR
     rep_origin      4368..4823
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4455..4474)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4665..4686
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4840..4859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4959..4981
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5019..5037)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5105..5209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5210..5278,5279..6070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5428..5447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6241..6829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6730..6749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaccat caccaaggca gttctcatta
      181 aaaaaaaaaa aacatttaat tgggagcttt cttacagttt cagagatgag taaattatta
      241 ccataatcag gagtgttgtg gcagacacag gcttgatgct ggagccatag ctgagagcta
      301 tatgctgatc cacagacaac agacacagac agacagacag acagacagac agacagactg
      361 actggctggc tggctggctg gctggctggc tctgggcttg gcatggactt ttgaaccccc
      421 aaaacccacc ccagtgacgt gctttctcca acaaggccac acattctaat ccttttaatc
      481 tttccaaaca gcaccagtcc ctggtgacta agcattcaaa tatatgagcc ttcttattca
      541 gaccaccaaa attactttat ttttccacat gaaagtatta tgtggcttcc tgtctgcaaa
      601 gaggaagaca tccatgaaca ctaatgacac tgggtttggg ctatgtccgg agcagaggaa
      661 tgaggccatg tagactaaat atgtgcatgt ggaggaggct gaaaacacat cagagctagc
      721 gctgcaggaa atgcttctgc attgcatacc cagagtttcc ttgctcatct gggagtctgt
      781 gtttttccta gatgtgtgca cttgtgtgag attctctggg tgtgagtcaa agtgttatct
      841 gaatgtgtaa tgtgtgctca atatgctcat gtgtgttacc ctgagcttct gtgtctacat
      901 atatacctgg atgcctgtgt gttctgtgat gtacatatat attctgtctt tccttccttt
      961 tctatttgtg ttattccatg tgttctttca gattctcacc accaagggca aggatatgtt
     1021 aactacccaa gtgtccacct ccgcctgtct ggtgatgttt acgccacccc cgtgctcttt
     1081 tctttgcccg acagagttgt tataggagat gtctccccgg gaacactgca ggaaggagaa
     1141 tttctacatt tatgttcccc tctgagtgtg cttctatccc caaaatgcct tcaaaggtga
     1201 aaatcaacac tggaaaccca agtatctggg aagggcaaga gtgtgtaagt gcaagttagc
     1261 ctaaggaata ggaagaggtt ggtaaacagg gtaggatcgt gggagggagt ttcgttacta
     1321 caggtccgga ccctcaggac aagaacccca cccccactcc ccaaattgcg catcccccgc
     1381 ccccatcaga gggggagggg aagaggttgc ggcgcggcgc atgcgcactg tcggattcag
     1441 caccgcggtc agagccttcg cctccgctgc cggcgcgcac caccacctcc ccagcaccaa
     1501 aggctgactg acgtcactca ctagccctcc ccaaactccc cttcctcgcc gccttggtcg
     1561 cgtccatgct gccgtgagtc cagtcggacc gcaccacgag aggtgcaaga tagggaggtg
     1621 cgggcgcgac catacgctct gcggcggcag agcctcggcg ctgcctcagt ctgcagcggg
     1681 cagcagagga gtcgcgtcgt gccagagagc accgccgtgc tcctgagccc ctcgcgctcc
     1741 gcccccgcgg cccacagacc cactgcccct tggaattccc cgatctgcac cggtcgccac
     1801 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1861 cggcgacgta aacggccaca agttcagcgt gaggggcgag ggcgagggcg atgccaccaa
     1921 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1981 cctcgtgacc accctgagcc acggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     2041 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2101 cttcaaggac gacggcacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2161 ggtgaaccgc atcgagctga agggcgtcga cttcaaggag gacggcaaca tcctggggca
     2221 caagctggag tacaacttca acagccacaa catctatatc atggccgtca agcagaagaa
     2281 cggcatcaag gtgaacttca agatccgcca caacgtggag gacggcagcg tgcagctcgc
     2341 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacagcca
     2401 ctacctgagc acccagtccg tgctgagcaa agaccccaac gagaagcgcg atcacatggt
     2461 cctgctggag ttccgcaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
     2521 ttccggttcc atggccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga
     2581 tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt
     2641 ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa
     2701 gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata
     2761 tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat
     2821 gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt cactggttat
     2881 gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt
     2941 cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc gctgccagga
     3001 tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat
     3061 tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat
     3121 tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt
     3181 aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc cgaataacta
     3241 cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc
     3301 aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga
     3361 tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg
     3421 agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa
     3481 tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg
     3541 cctgctggaa gatggcgatt aagccattaa cgcgggggat cctctagagt cgacctgcag
     3601 aagcttgcct cgagcagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc
     3661 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     3721 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     3781 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     3841 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     3901 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     3961 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     4021 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     4081 caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga
     4141 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     4201 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     4261 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     4321 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     4381 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4441 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4501 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4561 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     4621 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4681 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     4741 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4801 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     4861 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4921 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4981 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5041 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5101 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5161 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5221 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5281 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5341 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5401 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5461 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5521 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5581 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5641 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5701 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5761 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5821 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5881 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5941 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6001 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6061 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6121 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6181 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6241 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6301 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6361 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     6421 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6481 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6541 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6601 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6661 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6721 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6781 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6841 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
