LOCUS       sequence-78680-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length PFB0475c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: PFB0475c-bio-His
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     205..224
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    223..278
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(330..347)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agaacccctt tgtgtttggt ccccccccgt gtcacatgtg gaacagggcc cagttggcaa
       61 gttgtaccaa ccaactgaag ggattacatg cactgccccg cgaagaaggg gcagagatgt
      121 cgtagtcagg tttagttcgt ccggggcggg gatcgatcct ctagctagag atctccgagg
      181 gatctcgacc aaatgatttg ccctcccata tgtccttccg agtgagagac acaaaaaatt
      241 ccaacacact attgcaatga aaataaattt cctttattag ccagaggtcg aggtcggggg
      301 atccttaatt aatcacgtgt ctagactcaa tggtggtgat ggtggtgggg gcccagaggg
      361 gccagaggag gcaggttcct gtccctgtgg ttccacagca tcttctgggc gtccaggatg
      421 tggtgcaggc tgccggaatt caaccctttg gataaaacct ggatcttgga gtccatcttg
      481 acctcttcac cttcactcag tagacattgc cacacccctg cttcaggggc ctgcacttga
      541 atcactttct cctgcctgga gaccctggcc tcctgattct cctgcttcaa gatcagtctc
      601 atcttgggtg aggtgggtcc catcacctca caggtcaaag tgttgctgtc gggctgagtc
      661 actttcatca ccaccaggtt cacttcctga tacagtatcc ctctgtccag agtcagggtc
      721 aggttgccag aaccagcaaa ctgaagggag acctggggta tctgaagggt gagtgggagc
      781 gtttcggaca gctggaactt ggggttgcta gtagacttct gcacagacac cttttggttc
      841 tttagggaga aggtgatcca ggactgggaa gaaggagcct tctctgcctt ccatctcaac
      901 tctccctgca ggctttcctc tccaaggt
//
