LOCUS       sequence-78687-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length PFA0135w.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: PFA0135w-bio-His
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..229
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    228..283
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(335..352)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtcagagaac ccctttgtgt ttggtccccc cccgtgtcac atgtggaaca gggcccagtt
       61 ggcaagttgt accaaccaac tgaagggatt acatgcactg ccccgcgaag aaggggcaga
      121 gatgtcgtag tcaggtttag ttcgtccggg gcggggatcg atcctctagc tagagatctc
      181 cgagggatct cgaccaaatg atttgccctc ccatatgtcc ttccgagtga gagacacaaa
      241 aaattccaac acactattgc aatgaaaata aatttccttt attagccaga ggtcgaggtc
      301 gggggatcct taattaatca cgtgtctaga ctcaatggtg gtgatggtgg tgggggccca
      361 gaggggccag aggaggcagg ttcctgtccc tgtggttcca cagcatcttc tgggcgtcca
      421 ggatgtggtg caggctgccg gaattcaacc ctttggataa aacctggatc ttggagtcca
      481 tcttgacctc ttcaccttca ctcagtagac attgccacac ccctgcttca ggggcctgca
      541 cttgaatcac tttctcctgc ctggagaccc tggcctcctg attctcctgc ttcaagatca
      601 gtctcatctt gggtgaggtg ggtcccatca cctcacaggt caaagtgttg ctgtcgggct
      661 gagtcacttt catcaccacc aggttcactt cctgatacag tatccctctg tccagagtca
      721 gggtcaggtt gccagaacca gcaaactgaa gggagacctg gggtatctga agggtgagtg
      781 ggagcgtttc ggacagctgg aacttggggt tgctagtaga cttctgcaca gacacctttt
      841 ggttcttta
//
