LOCUS       sequence-78733-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon 
            configuration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pETM6-FAS
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gatgcgtccg gcgtagccta ggatcgagat cgatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atatacatat ggttgcgtct attgccgcct cagccttctt cccgaccccg
      181 tcaagcagca gttcagcagc atccgctaaa gcctctaaaa ccattggcga aggcccgggt
      241 tctctggatg ttcgtggcat tgtcgcaaaa ccgaccagct ctagtgcggc catgcaggaa
      301 aaagttaaag tccaaccggt gccgaaaatc aacggcgcca aagtgggtct gaaagttgaa
      361 acccagaaag cagatgaaga atcctcaccg tcgagcgctc cgcgtacgtt ttataatcaa
      421 ctgccggact ggagcgtgct gctggcagct gttaccacga ttttcctggc ggccgaaaaa
      481 cagtggaccc tgctggattg gaaaccgcgt cgcccggata tgctggcgga cgcatttggc
      541 ctgggtaaaa ttgtccagga tggcctggtg ttcaaacaaa acttctccat ccgttcatac
      601 gaaatcggtg cagaccgcac cgcgagcatc gaaacgctga tgaaccatct gcaggaaacc
      661 gcgctgaatc acgtgaaatc tgcaggtctg atgggtgatg gtttcggtgc aaccccggaa
      721 atgagtaaac gtaacctgat ttgggtggtt
//
