LOCUS       sequence-78742-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 L671C mutant with an N-terminal 3xFLAG-YFP tag
            in mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  TITLE     Forbush Lab human NKCC1 NT clones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 L671C (NT440)
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tatgaataga aggtaaggac actctgatga ttcccacgaa ctagtaggat tggtggtaga
       61 tccttagata gtgcttctaa ccatgccatg tagagagcac tagaaacggc accctttcgt
      121 gcgaccggta aactcatcac aataatgtta gctgtgcttg aatgttcctt taataactca
      181 tttaacctga tctgccggta tgtcttggtc ttatacagct ccaattcatt atctgttatt
      241 cgccagggtt catcttcttt cattttatct gcgatatctt gctctttatc atcttcgtga
      301 agtctgtatg gctcaatgat ttcctcgaaa gctataatgt tttctttctt tggtttggta
      361 ttgatgtctc ctagaaccat gatgtcagaa aaatcgattc tgaacttgct cagtaaagta
      421 gccatcgctc tccggtcatg gtctattctg tttatctttc caccaatgaa tactctgatc
      481 ttacagtctt tccatttttt cttggtcgtc agaaggtaag gtatcaataa ggtcagacct
      541 ccgtcatcaa aaagccacca gacatcaata gtattctttc cttgtttttt ctgaaactgt
      601 gtactagctt caagaagctt ttggtcagct acattcaaag gcacaatagg gcctttggat
      661 tcttttttca acagtggttg agttgcagtc ttgccatctt cttcctcgac tttatgggta
      721 attggttttt cactgagggg tttcgaagta tctaaatcgg actttttact atattccaca
      781 cttactacga catccttggt gccaggagat ttctcttgtg atgacaataa ttcttcttgt
      841 ccctgcaggt gactaatatc cagaccttct tttaggcgaa taaccactac gccatattgt
      901 atgtcaaaag catcgtgaaa taagtttatg tacatatcca catccctcat atctgcttgc
      961 aaccaatctt tct
//
