LOCUS       sequence-78765-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Mues M, Bartholomaus I, Thestrup T, Griesbeck O, Wekerle H, Kawakami
            N, Krishnamoorthy G
  TITLE     Real-time in vivo analysis of T cell activation in the central 
            nervous system using a genetically encoded calcium indicator.
  JOURNAL   Nat Med. 2013 Jun;19(6):778-83. doi: 10.1038/nm.3180. Epub 2013 May 
            12.
  PUBMED    23685843
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nm.3180"; 
            journalName: "Nat Med"; date: "2013-06"; volume: "19"; issue: "6"; 
            pages: "778-83"
COMMENT     Sequence Label: Twitch-1 pRSETB
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..763)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="GGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVRGE
                     GEGDATNGKLTLKFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEG
                     YVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNV
                     YIMADKQKNGIKANFKIRHNIE"
     primer_bind     225..244
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     484..505
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(611..632)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agcagccgga tcaagcttcg aattcttagt cctcgatgtt gtggcggatc ttgaagttgg
       61 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
      121 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      181 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      241 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      301 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca catcaggccg tagcccaggg
      361 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
      421 tcagcttgcc gttggtggca tcgccctcgc cctcgccgcg cacgctgaac ttgtggccgt
      481 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      541 tgctcaccat gctgccgccg gtaccgccct tgtacagctc gtccatgccg agagtgatcc
      601 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      661 tcagcttgga ctggtagctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
      721 tgggggtgtt ctgctggtag tggtcggcga gctgcacgcc gcc
//
