LOCUS       sequence-78797-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 
            promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSN6I01
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..53)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(60..80)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(87..272)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             complement(309..329)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(345..365)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(384..431)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             511..519
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acttgtatgg aggcctgagc tcatgaggcg tagtcaggga catcgtatgg gtaggagccg
       61 accttccgct tcttcttagg ggacccgggc tcctcgccct tctccaggcg gagaataaca
      121 tccggcttcg tcagctggta gccgagggac acgaggttct tgtagttctc cagcatcacg
      181 ttccggtaaa cgatctgctg agcagtgtcc aggagcttcc actcctccct tgtgaaatcg
      241 acgaacacgt ccttgaacgt caccagggtg cggctccaag cggtgaggga cttagcgtcc
      301 atcgagccga ccttccgctt cttctttggt gacccgcttg agccgacctt ccgcttcttc
      361 tttggcgacc ccgaggaccc ggacttcttc ttcttcgcct gccccgcctt cttcgtcgcc
      421 gctggccgct tgtcgccccc gagctgagac aggtcgatgc gcgtctcgta gaggccggta
      481 atcgactggt ggatgagggt cgcgtccagg acctccttag tgcttgtgta cctcttgcga
      541 tcgatagttg tgtcgaagta cttgaaagca gcaggggcgc cgaggttcgt cagggtgaag
      601 agatgaatga tattctcagc ctgctccctg attggcttgt cgcggtgctt gttgtacgcg
      661 gagaggacct tatccagatt cgcgtcggcc aggatcacgc gcttggagaa ctcggaaatc
      721 tgctcaatga tctcgtcgag gtaatgcttg tgctgctcga cgaacagctg cttctgctcg
      781 ttgtcctcgg ggctgccctt gagcttctcg tagtgggagg ccaggtagag gaagttcaca
      841 tacttggacg gcaga
//
