LOCUS       sequence-78854-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato 
            between AAV2 ITR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Lin JY, Lin MZ, Steinbach P, Tsien RY
  TITLE     Characterization of engineered channelrhodopsin variants with 
            improved properties and kinetics.
  JOURNAL   Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034.
  PUBMED    19254539
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2008.11"; journalName: "Biophys J"; date: "2009-03-4-
            4"; volume: "96"; issue: "5"; pages: "1803-14"
COMMENT     Sequence Label: AAV-oChIEF-tdTomato
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(130..153)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(328..347)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     597..615
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gaggttgatt atcgataagc ttgatatcga attcggggct agcctacttg tacagctcgt
       61 ccatgccgta caggaacagg tggtggcggc cctcggagcg ctcgtactgt tccacgatgg
      121 tgtagtcctc gttgtgggag gtgatgtcca gcttggtgtc cacgtagtag tagccgggca
      181 gttgcacggg cttcttggcc atgtagatgg tcttgaactc caccaggtag tggccgccgt
      241 ccttcagctt cagggcctgg tggatctcgc ccttcagcac gccgtcgcgg gggtacaggc
      301 gctcggtgga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggggggga
      361 agttggtgcc gcgcatcttc accttgtaga tcagcgtgcc gtcctgcagg gaggagtcct
      421 gggtcacggt caccagaccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
      481 cggggaagga cagcttcttg taatcgggga tgtcggcggg gtgcttcacg tacgccttgg
      541 agccgtacat gaactggggg gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg
      601 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
      661 tctcgaactc gtggccgttc atggagccct ccatgcgcac cttgaagcgc atgaactctt
      721 tgatgacggc catgttgttg tcctcggagg aggcggtgcc ggagctgccg ctgccggtgc
      781 tgccggtgc
//
