LOCUS       sequence-78861-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV2 transfer vector containing the InSynC 
            (SYP1-miniSOG-T2A-mCherry) construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Lin JY, Sann SB, Zhou K, Nabavi S, Proulx CD, Malinow R, Jin Y, 
            Tsien RY
  TITLE     Optogenetic inhibition of synaptic release with chromophore-assisted
            light inactivation (CALI).
  JOURNAL   Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022.
  PUBMED    23889931
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.05"; journalName: "Neuron"; date: 
            "2013-07-24- 24"; volume: "79"; issue: "2"; pages: "241-53"
COMMENT     Sequence Label: AAV-SYP1-miniSOG-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..757)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(325..344)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     594..612
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(764..817)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcttac ttgtacagct
       61 cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt
      121 agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct
      181 gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct
      241 tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct
      301 cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt
      361 tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg
      421 tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
      481 ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc
      541 cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca
      601 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
      661 cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga
      721 tgatggccat gttatcctcc tcgcccttgc tcaccatcgt acgagggccg ggattctcct
      781 ccacgtcacc gcatgttaga agacttcctc tgccctcacc gccaccagat ccacctccgc
      841 tagctccatc cagctgcact ccgatgaaat actggagctc acc
//
