LOCUS       sequence-78865-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flexed-ReaChR-citrine in AAV2 vector under human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Lin JY, Knutsen PM, Muller A, Kleinfeld D, Tsien RY
  TITLE     ReaChR: a red-shifted variant of channelrhodopsin enables deep 
            transcranial optogenetic excitation.
  JOURNAL   Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 
            2013 Sep 1.
  PUBMED    23995068
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3502"; 
            journalName: "Nat Neurosci"; date: "2013-10"; volume: "16"; issue: 
            "10"; pages: "1499-508"
COMMENT     Sequence Label: AAV-flex-ReaChR-citrine
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(131..164)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcataa cttcgtatag
       61 catacattat acgaagttat tctttgcacc attctaaaga ataacagtga taatttctgg
      121 gttaaggcaa ataacttcgt ataggatact ttatacgaag ttatgctagc accatggtga
      181 gcagaagacc ctggctgctg gccctggccc tggccgtggc cctggccgcc ggcagcgccg
      241 gcgccagcac cggcagcgac gccaccgtgc ccgtggccac ccaggacggc cccgactacg
      301 tgttccacag agcccacgag agaatgctgt tccagaccag ctacaccctg gagaacaacg
      361 gcagcgtgat ctgcatcccc aacaacggcc agtgcttctg cctggcctgg ctgaagagta
      421 acggcaccaa cgccgagaag ctggccgcca acatcctgca gtgggtggtg tttgcgctga
      481 gcgtggcgtg cctgggctgg tatgcgtatc aggcgtggcg cgcgacctgc ggctgggaag
      541 aagtgtatgt ggcgctgatt gaaatgatga aaagcattat tgaagcgttt catgaatttg
      601 atagcccggc gaccctgtgg ctgagcagcg gcaacggcgt ggtgtggatg cgctatggcg
      661 aatggctgct gacctgcccc gtgattctga ttcatctgag caacctgacc ggcctgaaag
      721 atgattatag caaacgcacc atgggcctgc tggtgagcga cgtgggctgc attgtgtggg
      781 gcgcgaccag cgcgatgtgc accggctgga ccaaaattct gttttttctg at
//
