LOCUS       sequence-78924-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB67
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(247..265)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(286..302)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(286..302)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(299..321)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    310..326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(334..340,341..358,359..364))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    379..400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(517..534)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caatctctct tcaattccat tttgaagtgc tttaaacccg aaaggttgag aaaaatgcga
       61 gcgctcaaat atttgtattg tgttcgttga gtgacccaac aaaaagagga aactttattg
      121 tgccgccaag aaaaaagtct cattgcctaa gagaaacata tgtggttgga aatcttccag
      181 atcgtggatc actagtatgt ggttggaaat cttccagatc gtggatcaga gctccagctt
      241 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
      301 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      361 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      421 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      481 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      541 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      601 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      661 ccgtaaaaag gccgcgttgc tggcgttttt c
//
