LOCUS       sequence-78948-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato 
            between AAV2 ITR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Lin JY, Lin MZ, Steinbach P, Tsien RY
  TITLE     Characterization of engineered channelrhodopsin variants with 
            improved properties and kinetics.
  JOURNAL   Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034.
  PUBMED    19254539
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2008.11"; journalName: "Biophys J"; date: "2009-03-4-
            4"; volume: "96"; issue: "5"; pages: "1803-14"
COMMENT     Sequence Label: AAV-oChIEF-tdTomato
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(91..114)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(289..308)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     558..576
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tgccgctgcc ggtgctgccg gtgccatgcc ccaggaacag gtggtggcgg ccctcggagc
       61 gctcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt
      121 ccacgtagta gtagccgggc agttgcacgg gcttcttggc catgtagatg gtcttgaact
      181 ccaccaggta gtggccgccg tccttcagct tcagggcctg gtggatctcg cccttcagca
      241 cgccgtcgcg ggggtacagg cgctcggtgg aggcctccca gcccatggtc ttcttctgca
      301 ttacggggcc gtcggggggg aagttggtgc cgcgcatctt caccttgtag atcagcgtgc
      361 cgtcctgcag ggaggagtcc tgggtcacgg tcaccagacc gccgtcctcg aagttcatca
      421 cgcgctccca cttgaagccc tcggggaagg acagcttctt gtaatcgggg atgtcggcgg
      481 ggtgcttcac gtacgccttg gagccgtaca tgaactgggg ggacaggatg tcccaggcga
      541 agggcagggg gccgcccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
      601 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt catggagccc tccatgcgca
      661 ccttgaagcg catgaactct ttgatgacct cctcgccctt gctcaccata tcgatgctgc
      721 tctcgtactt gccggtgccc ttgttcacgg cgccggcctc ggcctcgtcc tccacc
//
