LOCUS       sequence-78984-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 I674C I730C mutant with an N-terminal 
            3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing 
            convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Monette MY, Somasekharan S, Forbush B
  TITLE     Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion 
            Transport and Transporter Activation Revealed by Internal 
            Crosslinking between Transmembrane Domains 10 and 11/12.
  JOURNAL   J Biol Chem. 2014 Jan 22.
  PUBMED    24451383
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 Jan 22."
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835)
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgtgtccaca gatcatcaaa ccaacatttt ttgtgaaatc gtgaacaaga tgaagtaagg
       61 ccggccgtga gtttggcgct cctgtcataa caagacactg tggcctaaag tttttcacat
      121 ggtcctccac gccagaaagc ctaatactat gttgcagggc attgaggtaa gtcagggctt
      181 gtgtagagga tccccaattc acatcgggtt ttttgtaggt aacataaata tagagaccaa
      241 ggactatcac gtaggttagc aaagcagccc accagttaca gacgaacatt actatgcaac
      301 aaagaattgc tccaagaagt gagatccaca tattgtagta tttaaatgcc ggtcgccatc
      361 ctgggctttt ggccagtgaa gcatggaata ctgaaaaatt gatgagggca tagctggcaa
      421 ggaagaagtt tgagataatt ggtgcacaaa cattcagttc ggcaattaag atgaatccaa
      481 gtgcaattaa gaatgttaag atgtagccac gtaatggctc attatttttc ccataacctt
      541 tagcaaacat ctggaaagcg gggtagatgt tgtctttaca tagggcctga aatatcttgg
      601 gagcactcac tagggatgct aatgcgctag aaagagtggc agagaatatg cccgcggaaa
      661 ttagtggtgt aaatcctgac accatactca ttacctgaaa gttgttcatt aggccgtagc
      721 tacaaggact ggattcacaa gatgaaaaat caaagtttaa tttgcaggct gcgctagtac
      781 agtttgttag ctctgttacg atagtgtcat taacattacc agtggcatcg cgtacgacac
      841 aagaacctac agacacggca attcctacat aaaccaatgt agtaattaaa atggctagga
      901 gtgttccttt gggtatggct gactgaggat ctgca
//
