LOCUS       sequence-79061-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or 
            insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Kawaoka S, Hayashi N, Suzuki Y, Abe H, Sugano S, Tomari Y, Shimada 
            T, Katsuma S
  TITLE     The Bombyx ovary-derived cell line endogenously expresses 
            PIWI/PIWI-interacting RNA complexes.
  JOURNAL   RNA. 2009 Jul;15(7):1258-64. doi: 10.1261/rna.1452209. Epub 2009 May
            21.
  PUBMED    19460866
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1452209"; journalName: "RNA"; date: "2009-07"; volume: 
            "15"; issue: "7"; pages: "1258-64"
COMMENT     Sequence Label: pIZ-Flag6His-BmAgo3
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             570..593
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             594..611
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtatggtg ctaatgttgc ttcaacaaca attctgttga actgtgtttt catgtttgcc
       61 aacaagcacc tttatactcg gtggcctccc caccaccaac ttttttgcac tgcaaaaaaa
      121 cacgcttttg cacgcgggcc catacatagt acaaactcta cgtttcgtag actattttac
      181 ataaatagtc tacaccgttg tatacgctcc aaatacacta ccacacattg aacctttttg
      241 cagtgcaaaa aagtacgtgt cggcagtcac gtaggccggc cttatcgggt cgcgtcctgt
      301 cacgtacgaa tcacattatc ggaccggacg agtgttgtct tatcgtgaca ggacgccagc
      361 ttcctgtgtt gctaaccgca gccggacgca actccttatc ggaacaggac gcgcctccat
      421 atcagccgcg cgttatctca tgcgcgtgac cggacacgag gcgcccgtcc cgcttatcgc
      481 gcctataaat acagcccgca acgatctggt aaacacagtt gaacagcatc tgttcgaatt
      541 taaagcttgg taccgagctc ggatccatgg attacaagga tgacgacgat aagcatcatc
      601 atcatcatca tatggcagac ccaggcaaag gccgggggcg aagcttagcc cttttacagg
      661 cactgaagaa gtcacaaatg atggactctc cctcacaatc tgagagtcag tctcctgaaa
      721 gtacaccaga acagagtaca gctccaagta ccattgcaag tgcaactcct agtacttcag
      781 gagtgagtat aggcggtaga ggacgcgcag cagcattgat gctggctaaa atgcagcaga
      841 aaccaggctc cacaactcca gcaatttttg tgccaccttc atcaacttct gcaccaacag
      901 ctggaactgg acgtggattc aaattgcttc agaacttgca
//
