LOCUS       sequence-79128-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV2 transfer vector containing the InSynC 
            (SYP1-miniSOG-T2A-mCherry) construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Lin JY, Sann SB, Zhou K, Nabavi S, Proulx CD, Malinow R, Jin Y, 
            Tsien RY
  TITLE     Optogenetic inhibition of synaptic release with chromophore-assisted
            light inactivation (CALI).
  JOURNAL   Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022.
  PUBMED    23889931
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.05"; journalName: "Neuron"; date: 
            "2013-07-24- 24"; volume: "79"; issue: "2"; pages: "241-53"
COMMENT     Sequence Label: AAV-SYP1-miniSOG-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(113..166)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(194..511)
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct caccatcgta cgagggccgg
      121 gattctcctc cacgtcaccg catgttagaa gacttcctct gccctcaccg ccaccagatc
      181 cacctccgct agctccatcc agctgcactc cgatgaaata ctggagctca cccttctggt
      241 ccctcatggg ctggaggtgc aggaggttcc agaatttctt cccgcttttt gtgtagttta
      301 tcagctgcac ggtaatctcg cgctgatctc taatggcatc gcgaatcttc tgcactgtgg
      361 cctggtctgt ctctggtccc tgcagaaagc ggccattgcg gcccaggatc tcctctctgg
      421 aatactcggt cagctccagg aagccatcgg atgcgaagat gatgggattg tctggcagcc
      481 gtggatcagt tatcacgaaa cttttctcca tgctgccgcc accactgccg ccaccaccgg
      541 ttttcatctg attggagaag gaggtgggcg caccctgttg gccatagcct tgctgcccat
      601 agtcgccctg aggcccgtag ccaccgccac cgctggctgg ctgcccgtaa tcgggttgat
      661 aaccaccctg aggcccgtag gagtcctggg gcccatagcc tccggggccc tgcccgtagc
      721 ccgcatcgcc gtaggcatcg ccaggtgctg gttgcttttc cggggcgcct ggaggtgcgc
      781 gcatgaatgg ggctgcccag
//
