LOCUS       sequence-79142-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses proteorhodopsin using the Ppuf843-1200 promoter segment, 
            for use in R. sphaeroides .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Tikh IB, Held M, Schmidt-Dannert C
  TITLE     BioBrick compatible vector system for protein expression in 
            Rhodobacter sphaeroides.
  JOURNAL   Appl Microbiol Biotechnol. 2014 Feb 9.
  PUBMED    24509770
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2014 Feb 9."
COMMENT     Sequence Label: pBBRBB-Ppuf843-1200-PR
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(38..55)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagtgatggt gatggtgatg ctcgaggcgg ccgcttaatg gtgatgatgg tgatgagcat
       61 tagaagattc tttaacagca acattccata taattaaacc aaatagaatc ttgttaacaa
      121 agtcagcaag gttatagata aggtttaagt taagagctga tccaccgtca cccatcaggt
      181 aacctgtgaa ataacctaca ggataaatcg cccaaccaaa gatgataata tacatcattg
      241 tgttgtaagc tgattgcaca gcaggacttg cagtattaca tgcagatttt ccttctccag
      301 cccataattc ataaatcatg tatacccaag ctaaacaccc aataatgaat gcaggccatg
      361 cagccatgat tcctgcttca cccatgtaac caaacacaag cataacaaga gaaccaacta
      421 gtaatttctt aaataatgat ccagcaacat tagttgcagc agcaagaatt aagtagaatt
      481 cacatattaa tagaggaact gttagtaacc aatcaatgta tctaaataca gttggcgaat
      541 caccagtttc aatccatacc cctctcatgt acatgtaatg ccagaaagca ataccagtaa
      601 caagaccaga tacagttaat gatgttttcc attttgcaga aactctatct ctttcaacaa
      661 agaaaaatac agtagatgct aataaagcag cagtaactaa ccaaaaagaa acaccagtgt
      721 aatcactagc atcaaggtca ccaccacctg cagcaaatgt aggaagtgca ataacactac
      781 ctaatatcag taataatttc atagatctgc tatcctccgg atcgtaaga
//
