LOCUS       sequence-79143-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of the inactive mutant of mouse NRDc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Hiraoka Y, Matsuoka T, Ohno M, Nakamura K, Saijo S, Matsumura S, 
            Nishi K, Sakamoto J, Chen PM, Inoue K, Fushiki T, Kita T, Kimura T, 
            Nishi E
  TITLE     Critical roles of nardilysin in the maintenance of body temperature 
            homoeostasis.
  JOURNAL   Nat Commun. 2014;5:3224. doi: 10.1038/ncomms4224.
  PUBMED    24492630
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014"; volume: "5:3224. doi"; pages: " 
            10.1038/ncomms4224"
COMMENT     Sequence Label: pcDNA3.1-mNRDc-E>A-V5
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(30..71)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tcatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg
       61 ataggcttac cttcgaaggg ccctctagat ttgactattt tatggtaggg gaagaggctg
      121 agtgttgctg tgaaggccct gatgtcagta atgggggtgg tggattctgc caggacagga
      181 gagggtggca ggtaggttag ctgcatccct tcacgagaat ttgaatcctc accaaaaggg
      241 gcaccatcct cttccagttc atacttccca tatccaacaa catgaacgct gagcatttta
      301 cttccaggtc ctctgtgagc cttgaaccag ctgaccaggt ctgactttga gaaagacttc
      361 agggcttcaa tctcatgggc aaggcggtca aagagatact gctgtgtcac cacttcattc
      421 cagttcctgt ccacctcctc cccaaggtgg gtgtcctcac actctttcaa cttgatcagt
      481 gctgtgacct gagtattgaa tgcatcttct gtgaggttct caatcttctc ctcaaagctg
      541 gaaagaaatt cctctatctt cttgtcaaca gtttcggagt tgtatttggt tgcctgagtc
      601 ccaacagtta cagaaaatcc tagaattcca gatgtgttcc tacaggttgg gtagacatgg
      661 tacccaaggg tctgcttggt gcgaaggaag tcaaagcagg gctcttccat gtgcatcaca
      721 agcagctcca taagagtata ttctctcaga ctcctcgtac ctgactggta gtacacagtg
      781 acttcagagt tggcatcccc tttattcaga gctctgactt tgcacaggtg gtggccgctc
      841 ggcagctcca ccacctggaa ctgcacaggc atctccc
//
