LOCUS       sequence-79162-e         366 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for recombinant expression in bacteria of SUMO domain fused 
            to KTag, permitting covalent linkage to SpyTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Fierer JO, Veggiani G, Howarth M
  TITLE     SpyLigase peptide-peptide ligation polymerizes affibodies to enhance
            magnetic cancer cell capture.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Mar 17.
  PUBMED    24639550
REFERENCE   2  (bases 1 to 366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Mar 17."
COMMENT     Sequence Label: SUMO-KTag
FEATURES             Location/Qualifiers
     source          1..366
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtagca gccatcacca tcatcatcac gggagcgata gcgaagtgaa ccaggaagcg
       61 aaaccggaag ttaaaccgga agtgaaaccg gaaacccata ttaatctgaa agttagcgac
      121 ggcagcagcg aaatcttttt taaaattaaa aaaaccaccc cgctgcgtcg cctgatggaa
      181 gcctttgcga aacgtcaggg taaagaaatg gatagcctgc gctttctgta tgacggcatc
      241 cgtattcagg ccgatcagac cccggaagac ctggatatgg aagacaacga tattattgaa
      301 gcgcatcgcg aacagatcgg tggtggcgct acccatatta aattctcaaa acgtgatggt
      361 tattaa
//
