LOCUS       sequence-79211-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains fourth TALE repeat harboring NI as RVD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: p4NI
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(175..191)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(175..191)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(228..248)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(228..246)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(267..283)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(267..283)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(280..302)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    291..307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(315..321,322..339,340..345))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    360..381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(498..515)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(749..768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgggtctcg
       61 ctgaccccag cccaggttgt ggccatcgcc agcaacatag gtggcaagca ggccctcgaa
      121 accgtccaga gactgttacc ggttctctgc caggaccacg gcctgggaga ccgggatcca
      181 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
      241 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      301 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      361 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      421 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      481 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      541 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      601 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      661 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      721 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      781 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      841 tcgggaagcg tggcgctttc tcatagctca cgctgta
//
