LOCUS       sequence-79217-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed DsRed using the Ppuf1-1200 promoter segment, for use in R.
            sphaeroides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Tikh IB, Held M, Schmidt-Dannert C
  TITLE     BioBrick compatible vector system for protein expression in 
            Rhodobacter sphaeroides.
  JOURNAL   Appl Microbiol Biotechnol. 2014 Feb 9.
  PUBMED    24509770
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2014 Feb 9."
COMMENT     Sequence Label: pBBRBB-Ppuf1-1200-DsRed
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..162
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             239..256
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cccgcgacgg cgtgctgaag ggcgagatcc acaaggcgct gaagctgaag ggcggcggcc
       61 actacctggt ggagttcaag tcaatctaca tggccaagaa gcccgtgaag ctgcccggct
      121 actactacgt ggactccaag ctggacatca cctcccacaa cgaggactac accgtggtgg
      181 agcagtacga gcgcgccgag gcccgccacc acctgttcca gtaggcggcc gcctcgagca
      241 tcaccatcac catcactgat tctccttacg catctgtgcg gtatttcaca ccgcatgtac
      301 tagttaactt caggacctgc agtctagtat agtctggaac agcgcactta cgggttgctg
      361 cgcaacccaa gtgctaccgg cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc
      421 catcgtccag aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc
      481 attcctccgt ttcggtcaag gctggcaggt ctggttccat gcccggaatg ccgggctggc
      541 tgggcggctc ctcgccgggg ccggtcggta gttgctgctc gcccggatac agggtcggga
      601 tgcggcgcag gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca
      661 cggcggcact gaacaccgac aggcgcaact ggtcgcgggg ctggccccac gccacgcggt
      721 cattgaccac gtaggccgac acggtgccgg ggccgttgag cttcacgacg gagatccagc
      781 gctcggccac caagtccttg actgcgtatt ggaccgtccg caaagaacgt c
//
