LOCUS       sequence-79234-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for PCR-based transplant of Saccharomyces 
            cerevisiae TEF1 promoter in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Horecka J, Chu AM, Davis RW
  TITLE     IpO: plasmids and methods for simplified, PCR-based DNA transplant 
            in yeast.
  JOURNAL   Yeast. 2014 May;31(5):185-93. doi: 10.1002/yea.3006. Epub 2014 Mar 
            20.
  PUBMED    24604451
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3006"; 
            journalName: "Yeast"; date: "2014-05"; volume: "31"; issue: "5"; 
            pages: "185-93"
COMMENT     Sequence Label: pJH104
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        407..622
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 tatagggcga attgggtacc gggccccccc tcgagcgtac gctgcaggtc gacgaatcct
       61 tacatcacac ccaatccccc acaagtgatc ccccacacac catagcttca aaatgtttct
      121 actccttttt tactcttcca gattttctcg gactccgcgc atcgccgtac cacttcaaaa
      181 cacccaagca cagcatacta aattccccct ctttcttcct ctagggtggc gttaattacc
      241 cgtactaaag gtttggaaaa gaaaaaagag accgcctcgt ttctttttct tcgtcgaaaa
      301 aggcaataaa aatttttatc acgtttcttt ttcttgaaaa tttttttttt tgattttttt
      361 ctctttcgat gacctcccat tgattctaga agagtgcacc acgcttttca attcatcatt
      421 ttttttttat tctttttttt gatttcggtt tccttgaaat ttttttgatt cggtaatctc
      481 cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg
      541 tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac
      601 ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca
      661 tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa caaacttgtg
      721 tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag cat
//
