LOCUS       sequence-79236-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for PCR-based transplant of Saccharomyces 
            cerevisiae TEF1 promoter in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Horecka J, Chu AM, Davis RW
  TITLE     IpO: plasmids and methods for simplified, PCR-based DNA transplant 
            in yeast.
  JOURNAL   Yeast. 2014 May;31(5):185-93. doi: 10.1002/yea.3006. Epub 2014 Mar 
            20.
  PUBMED    24604451
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3006"; 
            journalName: "Yeast"; date: "2014-05"; volume: "31"; issue: "5"; 
            pages: "185-93"
COMMENT     Sequence Label: pJH104
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
       61 gtcatcgatg aattcgagct cgtttgtaat taaaacttag atagattgct atgctttctt
      121 tctaatgagc aagaagtaaa aaaagttgta atagaacaag aaaaacgaaa ctgaaacttg
      181 agaaattgaa gaccatttat taacttaaat atcaatggga ggtcatcgaa agagaaaaaa
      241 atcaaaaaaa aaaattttca agaaaaagaa acgtgataaa aatttttatt gcctttttcg
      301 acgaagaaaa agaaacgagg cggtctcttt tttcttttcc aaacctttag tacgggtaat
      361 taacgccacc ctagaggaag aaagaggggg aatttagtat gctgtgcttg ggtgttttga
      421 agtgggatcc tttcacaccg cagggtaata actgatataa ttaaattgaa gctctaattt
      481 gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc
      541 ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc
      601 ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat
      661 catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg
      721 gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa
      781 agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc
      841 cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct
      901 ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt
      961 gtgcatt
//
