LOCUS       sequence-79290-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses hCas9 under the CAG promoter for CRISPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Horii T, Tamura D, Morita S, Kimura M, Hatada I
  TITLE     Generation of an ICF syndrome model by efficient genome editing of 
            human induced pluripotent stem cells using the CRISPR system.
  JOURNAL   Int J Mol Sci. 2013 Sep 30;14(10):19774-81. doi: 
            10.3390/ijms141019774.
  PUBMED    24084724
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Int J Mol Sci"; date: "2013-09-30- 30"; volume: "14"; issue: "10"; 
            pages: "19774-81"
COMMENT     Sequence Label: pCAG-hCas9
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagtactcca ttgggctcga tatcggcaca aacagcgtcg gctgggccgt cattacggac
       61 gagtacaagg tgccgagcaa aaaattcaaa gttctgggca ataccgatcg ccacagcata
      121 aagaagaacc tcattggcgc cctcctgttc gactccgggg agacggccga agccacgcgg
      181 ctcaaaagaa cagcacggcg cagagatacc cgcagaaaga atcggatctg ctacctgcag
      241 gagatcttta gtaatgagat ggctaaggtg gatgactctt tcttccatag gctggaggag
      301 tcctttttgg tggaggagga taaaaagcac gagcgccacc caatctttgg caatatcgtg
      361 gacgaggtgg cgtaccatga aaagtaccca accatatatc atctgaggaa gaagcttgta
      421 gacagtactg ataaggctga cttgcggttg atctatctcg cgctggcgca tatgatcaaa
      481 tttcggggac acttcctcat cgagggggac ctgaacccag acaacagcga tgtcgacaaa
      541 ctctttatcc aactggttca gacttacaat cagcttttcg aagagaaccc gatcaacgca
      601 tccggagttg acgccaaagc aatcctgagc gctaggctgt ccaaatcccg gcggctcgaa
      661 aacctcatcg cacagctccc tggggagaag aagaacggcc tgtttggtaa tcttatcgcc
      721 ctgtcactcg ggctgacccc caactttaaa tctaacttcg acctggccga agatgccaag
      781 cttcaactga gcaaagacac ctacgatgat gatctcgaca atctgctggc ccagatcggc
      841 gaccagtacg cagacctttt tttggcggca aagaacctgt cagacgccat tctgctgagt
      901 gatattctgc gagtgaacac ggagatcacc aaagctccgc tga
//
