LOCUS       sequence-79302-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Ala.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli2 P5-6A
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..70
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             98..178
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             203..223
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agctcggatc cactagtcca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa
       61 aaagcaggct ggcgccggaa ccaattcagt cgacatgtac ccatacgatg ttccagatta
      121 cgcttatcct tatgacgtac ctgactatgc atacccttat gatgtaccag actacgctgg
      181 cgcggtacca agcggaggaa gcgagaatct ttattttcag ggcactggag gaacgagtcg
      241 acaaatggag acttctgccc cagcccctgc actggagaag aaagaagcca agagtggtct
      301 cttggaggac agcagcttcc ccgacccagg gaaaaaggcc tgtcctctgg cggtggccgc
      361 agctgtagcc gcccacggag ttcctcagca gctcctgccg gctttccacg cgcctttgcc
      421 gattgacatg agacaccagg agggaaggta ccattatgac cctcactctg tccacagtgt
      481 acacgggcct cccaccctaa gtggcagccc tgtcatctca gatatctcct tgatacgact
      541 ttctccacac cctgctggcc ctggagagtc acccttcagc gcccaccacc cctacgtgaa
      601 cccccatatg gagcactacc tccggtctgt gcacagcagc cccacactct caatgatctc
      661 tgccgccagg ggcctcagcc ctgctgatgt ggcccacgaa catctgaaag agaggggact
      721 ctttagcctc gcagccccag gcaccaaccc ttcagactat taccaccaga tgaccctcat
      781 ggcaagccac cccacccctt atgggga
//
