LOCUS       sequence-79304-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 nickase optimized for nuclear import..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins 
            A, Iyer V, Huang X, Skarnes WC
  TITLE     Efficient genome modification by CRISPR-Cas9 nickase with minimal 
            off-target effects.
  JOURNAL   Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857.
  PUBMED    24584192
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857."
COMMENT     Sequence Label: pST1374-N-NLS-flag-linker-Cas9-H840A
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             101..121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             131..154
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag caccatggga cctaagaaaa agaggaaggt
      121 ggcggccgct gactacaagg atgacgacga taaatctaga ctggaacccg gcgagaagcc
      181 atacaaatgc ccagagtgtg gtaaatcttt ctctcagtct ggtgctctga ccagacacca
      241 gcggacccac actagagaca agaaatactc tattggactg gatatcggga caaactccgt
      301 tggctgggcc gtcataaccg acgagtataa ggtgccaagc aagaaattca aggtgctggg
      361 taatactgac cgccattcaa tcaagaagaa cctgatcgga gcactcctct tcgactccgg
      421 tgaaaccgct gaagctactc ggctgaagcg gaccgcaagg cggagataca cccgccgcaa
      481 gaatcggata tgttatctgc aagagatctt tagcaacgaa atggctaagg tggacgactc
      541 cttctttcac cgcctggaag agagctttct ggtggaggag gataagaaac acgagaggca
      601 ccctatattc ggaaatatcg tggatgaggt ggcttaccat gaaaagtatc ctacaatcta
      661 ccatctgagg aagaagctgg tggacagcac cgataaagca gacctgaggc tcatctatct
      721 ggccctggct catatgataa agtttagagg acactttctg atcgagggcg acctgaatcc
      781 cgataattcc gatgtggata aactcttcat tcaactggtg cagacatata accaactgtt
      841 cgaggagaat cccataaacg cttctggtgt ggatgccaag gctattctgt ccgctcggct
      901 gtccaagtca cgcagactgg agaatctgat tgcccaactg ccaggagaaa
//
