LOCUS       sequence-79307-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 
            mutated to Glu.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli2 Pc-gE
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..74
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             102..182
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             207..227
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
       61 caaaaaagca ggctggcgcc ggaaccaatt cagtcgacat gtacccatac gatgttccag
      121 attacgctta tccttatgac gtacctgact atgcataccc ttatgatgta ccagactacg
      181 ctggcgcggt accaagcgga ggaagcgaga atctttattt tcagggcact ggaggaacga
      241 gtcgacaaat ggagacttct gccccagccc ctgcactgga gaagaaagaa gccaagagtg
      301 gtctcttgga ggacagcagc ttccccgacc cagggaaaaa ggcctgtcct ctggcggtgg
      361 ccgcagctgt agccgcccac ggagttcctc agcagctcct gccggctttc cacgcgcctt
      421 tgccgattga catgagacac caggagggaa ggtaccatta tgaccctcac tctgtccaca
      481 gtgtacacgg gcctcccacc ctaagtggca gccctgtcat ctcagatatc tccttgatac
      541 gactttctcc acaccctgct ggccctggag agtcaccctt cagcgcccac cacccctacg
      601 tgaaccccca tatggagcac tacctccggt ctgtgcacag cagccccaca ctctcaatga
      661 tctctgccgc caggggcctc agccctgctg atgtggccca cgaacatctg aaagagaggg
      721 gactctttag cctcgcagcc ccaggcacca acccttcaga ctattaccac cagatgaccc
      781 tcatggcaag ccaccccacc ccttatgggg accttctaat gcagagcggg
//
