LOCUS       sequence-79356-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses codon optimized human L1 retrotransposon driven by CMV 
            promoter and tagged with the self splicing intron neo cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Kroutter EN, Belancio VP, Wagstaff BJ, Roy-Engel AM
  TITLE     The RNA polymerase dictates ORF1 requirement and timing of LINE and 
            SINE retrotransposition.
  JOURNAL   PLoS Genet. 2009 Apr;5(4):e1000458. doi: 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24.
  PUBMED    19390602
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2009-04"; volume: "5(4):e1000458. doi"; pages: " 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24"
COMMENT     Sequence Label: L1-neo-TET
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatctcta gaagctgggt accagctgct agctaaaacc
       61 acaaagatgg gcaagaagca gaaccgcaag accggcaaca gcaagaccca gagcgccagc
      121 ccccccccca aggagcgcag cagcagcccc gccaccgagc agagctggat ggagaacgac
      181 ttcnacgagc tgcgcgagga gggnttccgc cgcagcaact acagcgagct gcgcgaggac
      241 atccagacca agggcaagga ggtggagaac ttcnagaana acctggagga gtgcatcacc
      301 cgcatcacca acaccgagaa gtgcctgaag gagctgatgg agctgaagac caaggnccgc
      361 gagctgcgcg aggagtgccg cagcctgngc agccgctgcg accagctgga ggagcgcgtg
      421 agcgcaatgg aggacganat gaacganatg aagcgcgagg gcaagttccg cgagaagcgc
      481 atcaagcgca acgagcagag cctccaggag atatgggact acgtgaagcg ccccaacctg
      541 cgcctgatcg gcgtgcccga nagcgacgtg gagaacggca ccaagctgga gaacacccta
      601 caggacatca tccaggagaa cttccccaac ctggcccgcc aggccaacgt gcagatccag
      661 gagatccagc gcacccccca
//
