LOCUS       sequence-79367-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4HSG
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtgacctcca ggaggatgag gatggtgttt
      121 atttcagctc atacgggcat tatgggatac atgaagaaat gctaaaggac aaaatacgaa
      181 cagaaagcta ccgagatttc atataccaaa atccacatat cttcaaagac aaggtagttt
      241 tggatgttgg gtgtggaact ggaattctct ctatgtttgc tgctaaagct ggggcgaaga
      301 aggttcttgg agttgatcaa tctgaaatac tttaccaggc aatggatatt ataagactaa
      361 ataaacttga agatactatt acactaatta aaggaaagat tgaagaagtt catcttcctg
      421 tagaaaaagt agatgttatc atatctgagt ggatgggcta ttttcttctg tttgagtcta
      481 tgttagattc tgtcctttat gcaaagaaca aatacttggc aaaaggaggc tcggtctacc
      541 ctgacatttg cactatcagc cttgtagcag tgagtgatgt gaataaacat gctgatagaa
      601 ttgctttttg ggatgatgtc tatggcttca agatgtcctg catgaagaaa gcagttattc
      661 cagaagctgt tgtggaagtt ttagatccga agactcttat ttcagaacct tgtggtatta
      721 agcatataga ttgccatacg acgtctatct cagatttgga attttcatca gattttaccc
      781 tgaaaatcac aaggacatcc atgtgcacgg caattgctgg ctactttgat atatattttg
      841 agaagaattg ccacaacagg gtcgtgttct ctacgggcc
//
