LOCUS       sequence-79424-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  adeno-associated virus (AAV) encoding a green fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Kaspar BK, Vissel B, Bengoechea T, Crone S, Randolph-Moore L, Muller
            R, Brandon EP, Schaffer D, Verma IM, Lee KF, Heinemann SF, Gage FH
  TITLE     Adeno-associated virus effectively mediates conditional gene 
            modification in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2320-5. Epub 2002 Feb 
            12.
  PUBMED    11842206
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-02-19"; volume: "99(4)"; pages: 
            "2320-5. Epub 2002 Feb 12"
COMMENT     Sequence Label: AAV GFP
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(18..20,21..23,24..734)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(18..20,21..23,24..734)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(63..84)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(324..343)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     671..692
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
       61 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      121 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      181 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      241 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      301 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      361 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      421 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      481 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      541 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      601 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
      661 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      721 acgagctgta caagtccgga ctcagatcct actagtagga tctcgagctc aagcttcgaa
      781 ttcaccccac cagtgcaggc tgcctatcag aaagtggtgg ctggtgtggc taatgccctg
      841 gcccacaagt atcactaagc tcgctttctt gctgtccaat ttctattaa
//
