LOCUS       sequence-79454-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length PFB0475c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: PFB0475c-bio-His
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     492..511
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      534..543
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
       61 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      121 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      181 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      241 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      301 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      361 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
      421 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
      481 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
      541 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcac
      601 tgtgcatctg cagaaacaga gagctgttcc cccacatcag cgagaacaag cccatcggcc
      661 agctgtaccg gctgatgaac atccacaaat acgagagctt cagcatcatc atccagatcc
      721 accacctgaa cctgaagttc ggcgacgacg acaacgccaa gttcatcgtg cacctgaaga
      781 tcggaaaccg ctacgcctac acccactacc acaagcagta ccagaac
//
