LOCUS       sequence-79507-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning insect cell expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Weinert C, Morger D, Djekic A, Grutter MG, Mittl PR
  TITLE     Crystal structure of TRIM20 C-terminal coiled-coil/B30.2 fragment: 
            implications for the recognition of higher order oligomers.
  JOURNAL   Sci Rep. 2015 Jun 4;5:10819. doi: 10.1038/srep10819.
  PUBMED    26043233
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-4"; volume: "5:10819. doi"; pages: " 
            10.1038/srep10819"
COMMENT     Sequence Label: pFBXC3GH
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(166..195)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(242..262)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagtact
      121 tctcgacaag cttgtcgaga ctgcaggctc tagatggtcg acttaatgat ggtgatgatg
      181 atggtgatga tgatgactag ttttgtagag ctcatccatg ccatgtgtaa tcccagcagc
      241 agttacaaac tcaagaagga ccatgtggtc acgcttttcg ttgggatctt tcgaaagggc
      301 agattgtgtc gacaggtaat ggttgtctgg taaaaggaca gggccatcgc caattggagt
      361 attttgttga taatggtctg ctagttgaac ggaaccatct tcaatgttgt ggcgaatttt
      421 gaagttagct ttgattccat tcttttgttt gtctgccgtg atgtatacat tgtgtgagtt
      481 atagttgtac tctagtttgt gtccgagaat gtttccatct tctttaaaat caataccctt
      541 taactcgata cgattaacaa gggtatcacc ttcaaacttg acttcagcac gcgtcttgta
      601 gttcccgtca tctttgaaag atatagtgcg ttcctgtaca taaccttcgg gcatggcact
      661 cttgaaaaag tcatgccgtt tcatgtgatc cggataacgg gaaaagcatt gaacaccata
      721 ggtcagagta gtgacaagtg ttggccaagg aacaggtagt tttccagtag tgcaaataaa
      781 tttaagggtg agttttccgt atgtagcatc accttcaccc tctccactga cagaaaattt
      841 gtgcccatt
//
