LOCUS       sequence-79509-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for 
            measurement of cotranslational protein degradation, use with 50571.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Turner GC, Varshavsky A
  TITLE     Detecting and measuring cotranslational protein degradation in vivo.
  JOURNAL   Science. 2000 Sep 22;289(5487):2117-20.
  PUBMED    11000112
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2000-09-22"; volume: "289(5487)"; pages: "2117-20"
COMMENT     Sequence Label: pDBD-M
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(746..772)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tacttcgcgt ctggccttcc tgtagccagc tttcatcaac attaaatgtg agcgagtaac
       61 aacccgtcgg attctccgtg ggaacaaacg gcggattgac cgtaatggga taggtcacgt
      121 tggtgtagat gggcgcatcg taaccgtgca tctgccagtt tgaggggacg acgacagtat
      181 cggcctcagg aagatcgcac tccagccagc tttccggcac cgcttctggt gccggaaacc
      241 aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      301 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagtcggg
      361 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      421 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc caagctccgg
      481 atcctgtcat accaccgcgg agccttagca caagatgtaa ggtggactcc ttctgaatgt
      541 tgtaatcaga cagcgttcta ccgtcttcta gctgcctacc ggcaaagatc aatctttgtt
      601 gatctggagg gataccttcc ttgtcttgaa ttttcgactt aacgttgtcg atggtatcgg
      661 aagattcaac ttccaatgtt atggttttac cggtcaaagt cttgacgaaa atctgcatgg
      721 tcgacccact acccgctaaa aatgcagcgt aatctggaac atcgtatggg ta
//
