LOCUS       sequence-79518-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes fabD, accA, accB, accC, accD in pseudo-operon 
            configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pACM4-ACA
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    145..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(169..188)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(170..188)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(226..270)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gcgtactgac tcttcgcctt cagaaatttc cagttcggag atgcctgatt cttcaaccag
       61 ctcgatcagt tttttaatct tacgaatatc catatgtatt atgtatatct ccttcttaaa
      121 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
      181 tcgtattaat ttcgcgggat cgagatcgat ctcgatccta gtagacgagt ccatgtgctg
      241 gcgttcaaat ttcgcagcag cggtttcttt accagactcg agttacgcgt aaccgtagct
      301 catcaggcgc tgataacgac gattttttaa atcttcagtg cttaacacgt cgagatcggc
      361 cagatccgcc agcagttgcg ctttcaacga tgccgccatc gcttccgggt tacggtgagc
      421 accacccagt ggttccggga tgatggagtc gatcagtttc agttctttca gacgcggagc
      481 aatgataccc atcgcttcag ccgccagcgg cgctttgtcg gcgctcttcc acagaatgga
      541 cgcacaacct tccggcgaga taacggaata ggtgctgtat tgcagcatat tcactttatc
      601 gcccacgcca atcgccagcg caccgccaga accaccttca ccgataaccg tacaaactac
      661 cggtacgccg aggcgagaca tttcacgcag gttgcgtgca atggcttcag actgaccacg
      721 ctcttctgcg cccacgccag gataagcccc cggggtgtcg ataaaggtga tgataggcat
//
