LOCUS       sequence-79543-e       10142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of TAL21 as input repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R
  TITLE     Accurate Predictions of Genetic Circuit Behavior from Part 
            Characterization and Modular Composition.
  JOURNAL   ACS Synth Biol. 2014 Nov 17.
  PUBMED    25369267
REFERENCE   2  (bases 1 to 10142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Nov 17."
COMMENT     Sequence Label: pExp_TRE:TAL21
FEATURES             Location/Qualifiers
     source          1..10142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        36..350
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    44..62
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    115..133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    151..169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    222..240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    258..276
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     323..347
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    423..447
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3836..3856
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3969..3993)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4083..4102)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4148..4203
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4202..4221)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4564..4580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4588..4604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4612..4618,4619..4636,4637..4642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4657..4678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4851..5687
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5717..5737)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5717..5735)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5756..5772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5780..5796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5804..5810,5811..5828,5829..5834))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5849..5870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5987..6004)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6158..6746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6238..6257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6917..7708,7709..7777))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7540..7559
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7778..7882)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7908..8363)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8045..8066)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8257..8276
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8490..8512
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8504..8521
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8505..8521
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8531..8550
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8531..8549
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8564..9367
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    9446..9471
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 caactttgta tagaaaagtt ggctccgaat ttctcgagtt tactccctat cagtgataga
       61 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
      121 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
      181 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
      241 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
      301 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagatttc
      361 gagctcggta cccggggatc ctctagtcag ctgacgcgtg ctagtggcgc gccgaattcg
      421 acccaagttt gtacaaaaaa gcaggctgat taccggagaa ttccaattga ccatgaccgg
      481 tttgtcgacc attcgtccgc gcaggccaag tcctgcccgc gagcttctgc ccggacccca
      541 accggatagg gttcagccga ctgcagatcg tggggtgtct gcgcctgctg gcagccctct
      601 ggatggcttg cccgctcggc ggacggtgtc ccggacccgg ctgccatctc cccctgcgcc
      661 ctcacctgcg ttctcggcgg gcagcttcag cgatctgctc cgtccgttcg atccgtcgct
      721 tcttgataca tcgcttcttg attcgatgcc tgccgtcggc acgccgcata cagcggctgc
      781 cccagcagag tgggatgagg cgcaatcggc tctgcgtgca gccgatgacc cgccacccac
      841 cgtgcgtgtc gctgtcactg ccgcgcggcc gccgcgcgcc aagccggccc cgcgacggcg
      901 tgctgcgcaa ccctccgacg cttcgccggc cgcgcaggtg gatctacgca cgctcggcta
      961 cagtcagcag cagcaagaga agatcaaacc gaaggtgcgt tcgacagtgg cgcagcacca
     1021 cgaggcactg gtgggccatg ggtttacaca cgcgcacatc gttgcgctca gccaacaccc
     1081 ggcagcgtta gggaccgtcg ctgtcacgta tcagcacata atcacggcgt tgccagaggc
     1141 gacacacgaa gacatcgttg gcgtcggcaa acagtggtcc ggcgcacgcg ccctggaggc
     1201 cttgctcacg gatgcggggg agttgagagg tccgccgtta cagttggaca caggccaact
     1261 tgtgaagatt gcaaaacgtg gcggcgtgac cgcaatggag gcagtgcatg catcgcgcaa
     1321 tgcactgacg ggtgcccccc tgaacctgac cccggaccaa gtggtggcta tcgccagcaa
     1381 caatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
     1441 ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg gcaagcaagc
     1501 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgactccgga
     1561 ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa gcgctcgaaa cggtgcagcg
     1621 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc
     1681 cagcaacaat ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
     1741 ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa
     1801 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
     1861 tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt
     1921 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc
     1981 tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
     2041 gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg
     2101 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
     2161 cctgactccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc aagcgctcga
     2221 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt
     2281 ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
     2341 gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa
     2401 caatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
     2461 ccatggcctg actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc
     2521 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga
     2581 ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg
     2641 gctgttgccg gtgctgtgcc aggaccatgg cctgactccg gaccaagtgg tggctatcgc
     2701 cagccacgat ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
     2761 ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa
     2821 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
     2881 cccggaccaa gtggtggcta tcgccagcaa cggtggcggc aagcaagcgc tcgaaacggt
     2941 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc
     3001 tatcgccagc aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
     3061 gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg
     3121 cggcaagcaa gcgctcgaaa gcattgtggc ccagctgagc cggcctgatc cggcgttggc
     3181 cgcgttgacc aacgaccacc tcgtcgcctt ggcctgcctc ggcggacgtc ctgccatgga
     3241 tgcagtgaaa aagggattgc cgcacgcgcc ggaattgatc agaagagtca atcgccgtat
     3301 tggcgaacgc acgtcccatc gcgttgccga ctacgcgcaa gtggttcgcg tgctggagtt
     3361 tttccagtgc cactcccacc cagcgtacgc atttgatgag gccatgacgc agttcgggat
     3421 gagcaggaac gggttggtac agctctttcg cagagtgggc gtcaccgaac tcgaagcccg
     3481 cggtggaacg ctccccccag cctcgcagcg ttgggaccgt atcctccagg catcagggat
     3541 gaaaagggcc aaaccgtccc ctacttcagc tcaaacaccg gatcaggcgt ctttgcatgc
     3601 attcgccgat tcgctggagc gtgaccttga tgcgcccagc ccaatgcacg agggagatca
     3661 gacgcgggca agcagccgta aacggtcccg atcggatcgt gctgtcaccg gcccctccgc
     3721 acagcaggct gtcgaggtgc gcgttcccga acagcgcgat gcgctgcatt tgcccctcag
     3781 ctggagggta aaacgcccgc gtaccaggat ctggggcggc ctcccggatc ccagccccaa
     3841 gaagaagaga aaggtggagg ccagcggtgg cggctcaaag cttggtggcg gctcaactag
     3901 ttaagggccc ggcgcgccta aggtaccccc gggtaactga aaatccaccg gatctagata
     3961 actgatctac ccagctttct tgtacaaagt ggtacgcgtg aattcactcc tcaggtgcag
     4021 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     4081 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     4141 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     4201 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     4261 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     4321 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     4381 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     4441 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     4501 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc
     4561 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4621 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4681 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatcgacag
     4741 tactaagctt ggtctaatac ccaatctctc gtcttatcca gatgttttat acgccttaat
     4801 taatagggat aacagggtaa tctcgaggtt taaacgtcga cgcggccgct actagggaca
     4861 ggattggtga cagaaaagcc ccatccttag gcctcctcct tcctagtctc ctgatattgg
     4921 gtctaacccc cacctcctgt taggcagatt ccttatctgg tgacacaccc ccatttcctg
     4981 gagccatctc tctccttgcc agaacctcta aggtttgctt acgatggagc cagagaggat
     5041 cctgggaggg agagcttggc agggggtggg agggaagggg gggatgcgtg acctgcccgg
     5101 ttctcagtgg ccaccctgcg ctaccctctc ccagaacctg agctgctctg acgcggctgt
     5161 ctggtgcgtt tcactgatcc tggtgctgca gcttccttac acttcccaag aggagaagca
     5221 gtttggaaaa acaaaatcag aataagttgg tcctgagttc taactttggc tcttcacctt
     5281 tctagtcccc aatttatatt gttcctccgt gcgtcagttt tacctgtgag ataaggccag
     5341 tagccagccc cgtcctggca gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc
     5401 ctttgtgaga atggtgcgtc ctaggtgttc accaggtcgt ggccgcctct actccctttc
     5461 tctttctcca tccttctttc cttaaagagt ccccagtgct atctgggaca tattcctccg
     5521 cccagagcag ggtcccgctt ccctaaggcc ctgctctggg cttctgggtt tgagtccttg
     5581 gcaagcccag gagaggcgct caggcttccc tgtccccctt cctcgtccac catctcatgc
     5641 ccctggctct cctgcccctt ccctacaggg gttcctggct ctgctctcca ccgcggtgga
     5701 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     5761 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5821 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5881 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5941 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6001 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6061 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6121 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6781 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6901 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6961 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7021 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7081 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7141 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7201 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7261 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7321 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7381 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7441 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7501 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7561 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7621 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7681 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7741 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7861 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     7921 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     7981 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     8041 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     8101 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     8161 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     8221 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     8281 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     8341 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     8401 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     8461 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     8521 tgagcgcgcg taatacgact cactataggg cgaattgggt acctgctttc tctgaccagc
     8581 attctctccc ctgggcctgt gccgctttct gtctgcagct tgtggcctgg gtcacctcta
     8641 cggctggccc agatccttcc ctgccgcctc cttcaggttc cgtcttcctc cactccctct
     8701 tccccttgct ctctgctgtg ttgctgccca aggatgctct ttccggagca cttccttctc
     8761 ggcgctgcac cacgtgatgt cctctgagcg gatcctcccc gtgtctgggt cctctccggg
     8821 catctctcct ccctcaccca accccatgcc gtcttcactc gctgggttcc cttttccttc
     8881 tccttctggg gcctgtgcca tctctcgttt cttaggatgg ccttctccga cggatgtctc
     8941 ccttgcgtcc cgcctcccct tcttgtaggc ctgcatcatc accgtttttc tggacaaccc
     9001 caaagtaccc cgtctccctg gctttagcca cctctccatc ctcttgcttt ctttgcctgg
     9061 acaccccgtt ctcctgtgga ttcgggtcac ctctcactcc tttcatttgg gcagctcccc
     9121 tacccccctt acctctctag tctgtgctag ctcttccagc cccctgtcat ggcatcttcc
     9181 aggggtccga gagctcagct agtcttcttc ctccaacccg ggcccctatg tccacttcag
     9241 gacagcatgt ttgctgcctc cagggatcct gtgtccccga gctgggacca ccttatattc
     9301 ccagggccgg ttaatgtggc tctggttctg ggtactttta tctgtcccct ccaccccaca
     9361 gtggggcaag cttattaccc tgttatccct aaccggtgcg tttttatgct tgtagtattg
     9421 tataatgttt ttaagatcca agcttctgac ctcttctctt cctcccacag ggcctcgaga
     9481 caattgcatc gatggtacca gtgaaagtct gcaatgaatt cgagttggcg cgcctgtcat
     9541 tctaaatctc tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct
     9601 caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg
     9661 gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc
     9721 ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt
     9781 tgggaggggg ctgtccccgt gagctcccag gcgcgcctgt cattctaaat ctctctttca
     9841 gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     9901 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
     9961 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
    10021 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc
    10081 cgtgagctcc caggcgcgcc aactcggtac cgtatcgatg tcgacgttaa cgctagtgat
    10141 at
//
